Journal: The FASEB Journal

Article Title: 12-HETER1/GPR31, a high-affinity 12( S )-hydroxyeicosatetraenoic acid receptor, is significantly up-regulated in prostate cancer and plays a critical role in prostate cancer progression

doi: 10.1096/fj.201500076

Figure Lengend Snippet: 12-HETER1 expression positively correlates with aggressiveness and progression of prostate tumors. A) Expression of 12-HETER1 in prostate carcinoma cells. Immunocytochemistry on HEK293 cells expressing 12-HETER1. a, b) Staining with 12-HETER1 antibody was optimized on HEK293 cells transfected with 12-HETER1 (a) or pcDNA (b) vector. Strong brown staining was detected in HEK293/12-HETER1 cells (a) compared to negative control (b). c, d) Immunohistochemistry to detect 12-HETER1 in 2 cases of human prostate cancer tissue. c) Benign gland (N, left corner, arrow) with rare cells showing positive staining, and neoplastic glands (T, GS-3/4, arrowhead) showing intense brown staining (arrowhead); original magnification, ×10. d) Strong staining in area (upper left, arrow) with mixed GS-3/4 tumor area, weak staining in area (lower right, arrowhead) with GS-3 tumor; original magnification, ×10. Images are representatives from 7 samples. B) Array analysis of 12-HETER1 expression. a, b) Immunohistochemistry to assay 12-HETER1 expression on 2 human prostate tissue microarrays. Expression of 12-HETER1 is significantly up-regulated in disease stage II/III human prostate tumor specimens (n = 8, P = 0.007, Student’s t test. c, d) Representative images of normal (c) and prostate tumor (d) specimens.

Article Snippet: Next we used qPCR to determine 12-HETER1 expression in 2 commercially available human prostate tissue microarrays from OriGene Technologies, HPRT101 and HPRT102 ( ).

Techniques: Expressing, Immunocytochemistry, Staining, Transfection, Plasmid Preparation, Negative Control, Immunohistochemistry

Journal: Heliyon

Article Title: Novel roles for HMGA2 isoforms in regulating oxidative stress and sensitizing to RSL3-Induced ferroptosis in prostate cancer cells

doi: 10.1016/j.heliyon.2023.e14810

Figure Lengend Snippet: HMGA2-WT and -TR Expression in PCa and Breast cancer Cell Lines and Patients Tissue (A) Western blot analysis shows the expression of HMGA2 protein in prostate cancer and breast cancer cell lines, HMGA2-WT displays a higher molecular weight at about 23 KDa while the HMGA2-TR displays a lower molecular of about 17 KDa. (B) Western blot analysis measured the protein levels of both HMGA2-WT and -TR in prostate cancer patient tissue displaying different Stage/Gleason scores. (C) RT-PCR measured the mRNA levels in cDNA array. (D) Immunohistochemical analysis was performed using prostate tissue microarray with HMGA2 antibody in normal prostate tissue, hyperplasia, and chronic inflammation prostate tissue. Scale bar; 100 μm. Magnification 20X, inset 40X. Statistical analysis was conducted utilizing GraphPad Prism; (**** p < 0.0001, ***p < 0.001, **p < 0.01). Bars represents SD of the mean. Alpha-tubulin was used as a loading control. Results are representative of 3 independent experiments.

Article Snippet: Prostate normal and cancer tissue cDNA array (TissueScan, Prostate Cancer cDNA Array III- CAT#: HPRT103) was purchased from OriGene (Rockville, MD).

Techniques: Expressing, Western Blot, Molecular Weight, Reverse Transcription Polymerase Chain Reaction, Immunohistochemical staining, Microarray, Control